《植物生理学报》 2010, 46(4): 395-398

禾本科植物叶片表皮气孔观察的样品制备方法改良

师长海^1,2, 李玉欣^1,2, 董宝娣¹, 乔匀周¹, 刘孟雨^1,*

1 中国科学院遗传与发育生物学研究所农业资源研究中心农业水资源重点实验室, 河北省节水农业重点实验室, 石家庄050021;2 中国科学院研究生院, 北京100049

通信作者：刘孟雨；E-mail: mengyuliu@ms.sjziam.ac.cn；Tel: 0311-85871 562

摘　要：

对现有的禾本科植物叶片气孔观察的样品制作方法中的不足作了一些改良。改良后的方法操作简便、耗时少、样品制备成功率高, 且放大后的效果好, 不会造成气孔形态的改变。改良方法适用于禾本科植物和其他叶肉紧实不易剥离的植物叶片。50% NaClO处理3 min 最适用于小麦旗叶表皮样品的制备。

关键词：禾本科植物; 叶片表皮; 气孔观察

收稿：2009-11-24   修定：2010-02-25

资助：国家‘‘863” 计划(2006AA100221)、国家科技支撑计划(2009BADA3B-03-08)、中国科学院创新方向性项目(KSCX2 -YW-N-0 0 4 )、中国科学院创新方向性项目( KS C X2 -YW-N - 0 4 2 - 0 1 ) 、国家自然科学基金(30870411)和国家‘‘ 十一五” 科技支撑计划(2008BAD95B13-04)。

An Improving Method of Sample Preparation for Epidermis Stoma Observation of Gramineous Plants Leaves

SHI Chang-Hai^1,2, LI Yu-Xin^1,2, DONG Bao-Di¹, QIAO Yun-Zhou¹, LIU Meng-Yu^1,*

¹Key Laboratory of Agricultural Water Resources & Hebei Key Laboratory of Agricultural Water-Saving, Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China;²Graduate School, Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: LIU Meng-Yu; E-mail: mengyuliu@ms.sjziam.ac.cn; Tel: 0311-85871 562

Abstract:

The method of sample preparation for epidermis stoma observation of gramineous plants leaves was improved. The improved method was easy to operate, timesaving. It also had high success rate, and kept the good shape of stoma after enlarged. The improved method was applicable to gramineous plants and other leaves with compacted mesophyll. The treatment of 50% NaClO for 3 min was best for obtaining epidermis sample of wheat boot leaf.

Key words: gramineous plants; leaf epidermis; stoma observation